Canine Pepsin A, PGA ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Canine PGA ELISA KIT , Canine PGNA ELISA KIT ,Canine Pepsin A ELISA Kit ,Canine Pepsin ELISA Kit ,Canine PGA5 ELISA KIT ,Canine pepsin A-5 ELISA KIT ,Canine PGA3 ELISA KIT ,Canine pepsinogen A5 ELISA KIT ,Canine pepsinogen-5 ELISA KIT ,Canine pepsinogen 5 ELISA KIT

Intended use

Canine PGA ELISA kit allows for the in vitro quantitative determination of Canine PGA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Canine PGA ELISA kit has been pre-coated with an antibody specific to PGA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

Canine PGA ELISA kit Manual download

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