Canine RPGR ELISA KIT

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  • Model: ELI-53170d
  • 20 Units in Stock
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Canine RPGR ELISA KIT

Product Name:Canine RPGR ELISA KIT
Packing:96T

Catalog No.:ELI-53170d

Gene Name:Canine RPGR

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine RPGR

Alternative Name:RPGR,RPGR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine RPGR ELISA KIT allows for the in vitro quantitative determination of Canine RPGR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine X- linked retinitis pigmentosa GTPase regulator, Rpgr ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

 

Alternative name

Canine Rpgr ELISA KIT ,Canine COD1 ELISA KIT ,Canine CORDX1 ELISA KIT ,Canine CRD ELISA KIT ,Canine PCDX ELISA KIT ,Canine RP15 ELISA KIT ,Canine RP3 ELISA KIT ,Canine XLRP3 ELISA KIT ,Canine orf15 ELISA KIT ,Canine retinitis pigmentosa 15 ELISA KIT ,Canine retinitis pigmentosa 3 GTPase regulator ELISA KIT ,Canine retinitis pigmentosa GTPase regulator ELISA KIT

 

Intended use

Canine Rpgr ELISA kit allows for the in vitro quantitative determination of Canine X- linked retinitis pigmentosa GTPase regulator concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Canine Rpgr ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rpgr, During the reaction, Rpgr in the sample or standard competes with a fixed amount of biotin-labeled Rpgr for sites on a pre-coated Monoclonal antibody specific to Rpgr. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rpgr in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine Rpgr ELISA kit manual request by email:sales@lifescience-market.com  

Order Canine Rpgr ELISA kit by email:sales@lifescience-market.com

has been pre-coated with an Canine RPGR antibody specific to Canine RPGR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine RPGR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine RPGR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine RPGR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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