Canine SULT1B1 ELISA Kit
Product Name:Canine SULT1B1 ELISA Kit
Packing:96T
Catalog No.:ELI-18146d
Gene Name:Canine SULT1B1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine SULT1B1
Alternative Name:SULT1B1,SULT1B1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine SULT1B1 ELISA Kit allows for the in vitro quantitative determination of Canine SULT1B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine SULT1B1 (Sulfotransferase family cytosolic 1B member 1) ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Canine SULT1B1 ELISA KIT ,Canine ST1B1 ELISA KIT ,Canine ST1B2 ELISA KIT ,Canine SULT1B2 ELISA KIT ,Canine sulfotransferase 1B1 ELISA KIT ,Canine sulfotransferase 1B2 ELISA KIT ,Canine sulfotransferase family ELISA KIT ,Canine cytosolic ELISA KIT ,Canine 1B ELISA KIT ,Canine member 1 ELISA KIT ,Canine thyroid hormone sulfotransferase ELISA KIT ,Canine sulfotransferase family 1B member 1 ELISA KIT
Intended use
Canine SULT1B1 ELISA kit allows for the in vitro quantitative determination of Canine Sulfotransferase family cytosolic 1B member 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Canine SULT1B1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SULT1B1, During the reaction, SULT1B1 in the sample or standard competes with a fixed amount of biotin-labeled SULT1B1 for sites on a pre-coated Monoclonal antibody specific to SULT1B1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SULT1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
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has been pre-coated with an Canine SULT1B1 antibody specific to Canine SULT1B1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine SULT1B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine SULT1B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine SULT1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!