Canine Telomerase reverse transcriptase, TERT ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TERT,CMM9; DKCA2; DKCB4; EST2; PFBMFT1; TCS1; TP2; TRT; hEST2; hTRT; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase,Telomerase reverse transcriptase,Telomerase-associated protein 2,TP2,HEST2,Telomerase catalytic subunit,

Search name

Canine TERT ELISA KIT ,Canine CMM9 ELISA KIT ,Canine DKCA2 ELISA KIT ,Canine DKCB4 ELISA KIT ,Canine EST2 ELISA KIT ,Canine PFBMFT1 ELISA KIT ,Canine TCS1 ELISA KIT ,Canine TP2 ELISA KIT ,Canine TRT ELISA KIT ,Canine hEST2 ELISA KIT ,Canine hTRT ELISA KIT ,Canine telomerase catalytic subunit ELISA KIT ,Canine telomerase-associated protein 2 ELISA KIT ,Canine telomerase reverse transcriptase ELISA KIT ,Canine Telomerase reverse transcriptase ELISA KIT ,Canine Telomerase-associated protein 2 ELISA KIT ,Canine TP2 ELISA KIT ,Canine HEST2 ELISA KIT ,Canine Telomerase catalytic subunit ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Telomerase reverse transcriptase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase reverse transcriptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Telomerase reverse transcriptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Telomerase reverse transcriptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Telomerase reverse transcriptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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