Canine terminal complement complex C5b-9, TCC C5b-9 ELISA Kit
Product Name:Canine terminal complement complex C5b-9, TCC C5b-9 ELISA Kit
Packing:96T
Catalog No.:ELA-E0796d
Gene Name:Canine terminal complement complex C5b-9, TCC C5b-9
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Canine terminal complement complex C5b-9, TCC C5b-9
Alternative Name:terminal complement complex C5b-9,terminal complement complex C5b-9, TCC C5b-9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine terminal complement complex C5b-9, TCC C5b-9 ELISA Kit allows for the in vitro quantitative determination of Canine terminal complement complex C5b-9, TCC C5b-9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine terminal complement complex C5b-9,TCC C5b-9 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
terminal complement complex C5b-9,TCC C5b-9, C5b-9, C5b9, TCC C5b9,
Search name
Canine terminal complement complex C5b-9 ELISA KIT ,Canine TCC C5b-9 ELISA KIT ,Canine C5b-9 ELISA KIT ,Canine C5b9 ELISA KIT ,Canine TCC C5b9 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine terminal complement complex C5b-9,TCC C5b-9 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canine terminal complement complex C5b-9,TCC C5b-9, During the reaction, Canine terminal complement complex C5b-9,TCC C5b-9 in the sample or standard competes with a fixed amount of biotin-labeled Canine terminal complement complex C5b-9,TCC C5b-9 for sites on a pre-coated Monoclonal antibody specific to Canine terminal complement complex C5b-9,TCC C5b-9. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Canine terminal complement complex C5b-9,TCC C5b-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine terminal complement complex C5b-9, TCC C5b-9 antibody specific to Canine terminal complement complex C5b-9, TCC C5b-9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine terminal complement complex C5b-9, TCC C5b-9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine terminal complement complex C5b-9, TCC C5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine terminal complement complex C5b-9, TCC C5b-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!