Canine TFRC ELISA KIT

Product Name:Canine TFRC ELISA KIT
Packing:96T

Catalog No.:ELI-02729d

Gene Name:Canine TFRC

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine TFRC

Alternative Name:Transferrin receptor protein 1,TFRC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine TFRC ELISA KIT allows for the in vitro quantitative determination of Canine TFRC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine TFRC ELISA KIT has been pre-coated with an Canine TFRC antibody specific to Canine TFRC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine TFRC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine TFRC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine TFRC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!