Canine Thiopurine S-methyltransferase, TPMT ELISA KIT

Product Name:Canine Thiopurine S-methyltransferase, TPMT ELISA KIT
Packing:96T

Catalog No.:ELI-03372d

Gene Name:Canine Thiopurine S-methyltransferase, TPMT

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Thiopurine S-methyltransferase, TPMT

Alternative Name:Thiopurine S-methyltransferase,Thiopurine S-methyltransferase, TPMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Thiopurine S-methyltransferase, TPMT ELISA KIT allows for the in vitro quantitative determination of Canine Thiopurine S-methyltransferase, TPMT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Thiopurine S-methyltransferase, TPMT ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Thiopurine S-methyltransferase,Thiopurine methyltransferase, TPMT,S-adenosyl-L-methionine:thiopurine S-methyltransferase

 

Search name

Canine Thiopurine S-methyltransferase ELISA KIT ,Canine Thiopurine methyltransferase ELISA KIT ,Canine TPMT ELISA KIT ,Canine S-adenosyl-L-methionine ELISA KI, Canine thiopurine S-methyltransferase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine thiopurine s-methyltransferase,TPMT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPMT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TPMT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TPMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Thiopurine S-methyltransferase, TPMT antibody specific to Canine Thiopurine S-methyltransferase, TPMT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Thiopurine S-methyltransferase, TPMT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Thiopurine S-methyltransferase, TPMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Thiopurine S-methyltransferase, TPMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!