Canine Thioredoxin, TXN ELISA KIT

Product Name:Canine Thioredoxin, TXN ELISA KIT
Packing:96T

Catalog No.:ELI-02438d

Gene Name:Canine Thioredoxin, TXN

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Thioredoxin, TXN

Alternative Name:Thioredoxin,Thioredoxin, TXN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Thioredoxin, TXN ELISA KIT allows for the in vitro quantitative determination of Canine Thioredoxin, TXN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Thioredoxin, TXN ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Thioredoxin(Trx),ATL-derived factor(ADF),Surface-associated sulphydryl protein(SASP), Thioredoxin,TRX; TRDX; TRX1; ADF;
ATL-derived factor; SASP; TXN delta 3; surface-associated sulphydryl protein; thioredoxin delta 3

Search name
Canine Thioredoxin ELISA Kit, Canine Trx ELISA Kit, Canine ATL-derived factor ELISA Kit, Canine ADF ELISA Kit, Canine
Surface-associated sulphydryl protein ELISA Kit, Canine SASP ELISA Kit, Canine TRDX ELISA Kit, Canine Trx1 ELISA Kit,
Canine TXN delta 3 ELISA Kit, Canine thioredoxin delta 3 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine thioredoxin,Trx concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trx. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Trx and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Trx, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Trx in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

has been pre-coated with an Canine Thioredoxin, TXN antibody specific to Canine Thioredoxin, TXN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Thioredoxin, TXN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Thioredoxin, TXN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Thioredoxin, TXN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!