Canine tissue inhibitors of metalloproteinase, TIMP1 ELISA Kit

Product Name:Canine tissue inhibitors of metalloproteinase, TIMP1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0552d

Gene Name:Canine tissue inhibitors of metalloproteinase, TIMP1

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine tissue inhibitors of metalloproteinase, TIMP1

Alternative Name:tissue inhibitors of metalloproteinase,tissue inhibitors of metalloproteinase, TIMP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine tissue inhibitors of metalloproteinase, TIMP1 ELISA Kit allows for the in vitro quantitative determination of Canine tissue inhibitors of metalloproteinase, TIMP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine tissue inhibitors of metalloproteinase, TIMP1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TIMP1,TIMP-1,CLGI; EPA; EPO; HCI; TIMP; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; metalloproteinase inhibitor 1; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1

 

Search name

Canine TIMP1 ELISA KIT ,Canine TIMP-1 ELISA KIT ,Canine CLGI ELISA KIT ,Canine EPA ELISA KIT ,Canine EPO ELISA KIT ,Canine HCI ELISA KIT ,Canine TIMP ELISA KIT ,Canine collagenase inhibitor ELISA KIT ,Canine erythroid potentiating activity ELISA KIT ,Canine erythroid-potentiating activity ELISA KIT ,Canine fibroblast collagenase inhibitor ELISA KIT ,Canine metalloproteinase inhibitor 1 ELISA KIT ,Canine tissue inhibitor of metalloproteinases 1 ELISA KIT ,Canine TIMP metallopeptidase inhibitor 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine TIMP-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metalloproteinase inhibitor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Metalloproteinase inhibitor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Metalloproteinase inhibitor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Metalloproteinase inhibitor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine tissue inhibitors of metalloproteinase, TIMP1 antibody specific to Canine tissue inhibitors of metalloproteinase, TIMP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine tissue inhibitors of metalloproteinase, TIMP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine tissue inhibitors of metalloproteinase, TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine tissue inhibitors of metalloproteinase, TIMP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!