Canine Vascuolar cell adhesion molecule 1, VCAM-1 ELISA kit

Product Name:Canine Vascuolar cell adhesion molecule 1, VCAM-1 ELISA kit
Packing:96T

Catalog No.:ELA-E0142d

Gene Name:Canine Vascuolar cell adhesion molecule 1, VCAM-1

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Canine Vascuolar cell adhesion molecule 1, VCAM-1

Alternative Name:Vascuolar cell adhesion molecule 1,Vascuolar cell adhesion molecule 1, VCAM-1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Vascuolar cell adhesion molecule 1, VCAM-1 ELISA kit allows for the in vitro quantitative determination of Canine Vascuolar cell adhesion molecule 1, VCAM-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Vascuolar cell adhesion molecule 1, VCAM- 1 ELISA kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vascular cell adhesion protein 1(V-CAM 1,VCAM-1),INCAM-100, VCAM-1,CD106; INCAM-100; CD106 antigen; vascular cell adhesion
protein 1; vascular cell adhesion molecule 1

Search name
Canine Vascular cell adhesion protein 1 ELISA Kit, Canine V-CAM 1 ELISA Kit, Canine Vascuolar cell adhesion molecule 1 ELISA
kit, Canine VCAM-1 ELISA kit, Canine INCAM-100 ELISA kit, Canine CD106 ELISA kit, Canine CD106 antigen ELISA kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine vascular cell adhesion protein 1,V-CAM 1 concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-CAM 1. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for V-CAM 1 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain V-CAM 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of V-CAM 1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Vascuolar cell adhesion molecule 1, VCAM-1 antibody specific to Canine Vascuolar cell adhesion molecule 1, VCAM-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Vascuolar cell adhesion molecule 1, VCAM-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Vascuolar cell adhesion molecule 1, VCAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Vascuolar cell adhesion molecule 1, VCAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!