SMD1168 chemically Yeast Express Competent Cells      

Product Name    SMD1168 chemically Yeast Express Competent Cells 

Packing 100*0.1ml

Alternative Name 

SMD1168 Competent Cells,SMD1168 chemically Yeast Express Competent Cells ,SMD1168 chemically Competent Cells 

SMD1168 Competent Cells Information

Strain type: Pichia pastoris
Culture medium: YPD medium
Growth conditions: 30 centigrade, aerobic
Genotypes: HIS4, pep4
Phenotypes: His-, pep4-, Mut+
Resistance: ampicin and kanana
Plasmids transformation conditions: electric conversion
Application: protein expression
Induction method: methanol

SMD1168 Description

SMD1168 is a Pichia pastoral strain, which belongs to eukaryotic cells. According to the general antibiotics such as prokaryotes and yeast to ampicillin kanamycin is invalid, so in order to prevent Escherichia coli and other prokaryotes strains pollution on yeast, often in the cultivation of adding some ampicillin and kanamycin antibiotic medium to inhibit bacterial growth and contamination.

The optimum growth temperature of Pichia pastoris at 28-30 degrees is 28-30 degrees, and it is generally used for 30 degrees. The temperature over 32 degrees may lead to cell death.

SMD1168 Pichia pastoris is a His nutritional deficiency strain, which can not be grown in the lack of histidine SC minimal media.

When making SMD1168 Pichia pastoris, please do not use the plate for more than 4 days to preserve the bacteria, and try to use the fresh plate as much as possible.

The carrier of SMD1168 is a yeast expression vector containing His4 gene, such as pPIC9K, pPIC3.5K.

The mutation of Pep4 gene in SMD1168 genome results in the loss of A activity of proteolytic enzymes, which can protect the expression products from degradation and promote the expression level.