Chicken 12- Hydroxyeicosatetraenoic acid, 12- HETE ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
12-Hydroxyeicosatetraenoic acid,12-HETE

Search name
Chicken 12- Hydroxyeicosatetraenoic acid ELISA Kit, Chicken 12- HETE ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of 12-hydroxyeicosatetraenoic acid,12-Hydroxyeicosatetraenoic
acid concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 12-Hydroxyeicosatetraenoic acid, During the reaction, 12-Hydroxyeicosatetraenoic acid in the
sample or standard competes with a fixed amount of biotin-labeled 12-Hydroxyeicosatetraenoic acid for sites on a pre-coated Monoclonal
antibody specific to 12-Hydroxyeicosatetraenoic acid. Excess conjugate and unbound sample or standard are washed from the plate.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution
is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-Hydroxyeicosatetraenoic acid in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

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