Chicken Early growth response protein 1,EGR1 ELISA Kit

Starting at: $796.00

  • Model: ELA-E0416c
  • 20 Units in Stock
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Chicken Early growth response protein 1, EGR1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Egr-1,AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225; nerve growth factor-induced protein A; transcription factor ETR103; transcription factor Zif268; zinc finger protein 225; zinc finger protein Krox-24; early growth response 1,Early growth response protein 1(EGR-1),; Zinc finger protein Krox-24,Zinc finger protein 225,Transcription factor Zif268,Transcription factor ETR103,AT225,Nerve growth factor-induced protein A(NGFI-A),;

 

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Chicken early growth response protein 1,EGR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EGR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EGR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EGR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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