Chicken Fibroblast growth factor receptor 3,FGFR3 ELISA KIT

Starting at: $796.00

  • Model: ELI-43995c
  • 20 Units in Stock
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Chicken FGFR3 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  FGFR3

Protein Name  Fibroblast growth factor receptor 3

Alternative Name

Chicken FGFR3 ELISA KIT ,Chicken ACH ELISA KIT ,Chicken CD333 ELISA KIT ,Chicken CEK2 ELISA KIT ,Chicken HSFGFR3EX ELISA KIT ,Chicken JTK4 ELISA KIT ,Chicken fibroblast growth factor receptor 3 ELISA KIT ,Chicken FGFR-3 ELISA KIT ,Chicken fibroblast growth factor receptor 3 variant 4 ELISA KIT ,Chicken hydroxyaryl-protein kinase ELISA KIT ,Chicken tyrosine kinase JTK4 ELISA KIT

Intended use

Chicken FGFR3 ELISA KIT allows for the in vitro quantitative determination of FGFR3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Chicken FGFR3 ELISA KIT has been pre-coated with an FGFR3 antibody specific to FGFR3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FGFR3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FGFR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FGFR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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