Chicken follicle-stimulating hormone, FSH ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Follitropin subunit beta,Follicle-stimulating hormone beta subunit(FSH-B,FSH-beta),Follitropin beta chain, Fshb,follicle-stimulating
hormone subunit beta; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; follicle -
stimulating hormone subunit beta; follicle stimulating hormone beta; follitropin beta chain; follitropin subunit beta; follicle stimulating
hormone, beta polypeptide

Search name
Chicken follicle-stimulating hormone ELISA Kit, Chicken Follitropin subunit beta ELISA Kit, Chicken FSH ELISA Kit, Chicken
Follicle-stimulating hormone beta subunit ELISA Kit, Chicken FSH-B ELISA Kit, Chicken FSH-beta ELISA Kit, Chicken Follitropin
beta chain ELISA Kit, Chicken follicle-stimulating hormone subunit beta ELISA Kit, Chicken follicle-stimulating hormone
beta-subunit ELISA Kit, Chicken FSHb ELISA Kit, Chicken follicle-stimulating hormone beta subunit ELISA Kit, Chicken follicle
stimulating hormone beta ELISA Kit, Chicken follitropin beta chain ELISA Kit, Chicken follitropin subunit beta ELISA Kit,
Chicken follicle stimulating hormone beta polypeptide ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of chicken Follitropin subunit beta concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follitropin subunit beta. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Follitropin subunit
beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain Follitropin subunit beta, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Follitropin subunit beta in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

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