Chicken Hemoglobin subunit alpha,HBA ELISA Kit

Starting at: $796.00

  • Model: ELA-E0517c
  • 20 Units in Stock
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Chicken Hemoglobin subunit alpha, HBA ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Hemoglobin subunit alpha,Alpha-globin,Hemoglobin alpha chain,HBA1,hemoglobin subunit alpha; HBH; alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; alpha-globin; delta globin; hemoglobin alpha 1 globin chain; hemoglobin alpha chain; hemoglobin alpha-1 chain; hemoglobin, alpha 1

 

Search name

Chicken Hemoglobin subunit alpha ELISA KIT ,Chicken Alpha-globin ELISA KIT ,Chicken Hemoglobin alpha chain ELISA KIT ,Chicken HBA1 ELISA KIT ,Chicken hemoglobin subunit alpha ELISA KIT ,Chicken HBH ELISA KIT ,Chicken alpha one globin ELISA KIT ,Chicken alpha-1 globin ELISA KIT ,Chicken alpha-1-globin ELISA KIT ,Chicken alpha-2 globin chain ELISA KIT ,Chicken alpha-globin ELISA KIT ,Chicken delta globin ELISA KIT ,Chicken hemoglobin alpha 1 globin chain ELISA KIT ,Chicken hemoglobin alpha chain ELISA KIT ,Chicken hemoglobin alpha-1 chain ELISA KIT ,Chicken hemoglobin alpha 1 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Chicken Hemoglobin subunit alpha concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin subunit alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hemoglobin subunit alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hemoglobin subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hemoglobin subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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