Chicken SELT ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name SELT
Protein Name Selenoprotein T
Alternative Name
Selenoprotein T,SELENOT,selenoprotein T; thioredoxin reductase-like selenoprotein T; SELT; thioredoxin reductase-like enzyme,
Intended use
Chicken SELT ELISA KIT allows for the in vitro quantitative determination of SELT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Chicken SELT ELISA KIT has been pre-coated with an SELT antibody specific to SELT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SELT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SELT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SELT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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