BUD13 ELISA KIT|Human
Catalog EF008178
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
BUD13 ELISA KIT Sensitivity< 0.094ng/ml
Application BUD13 ELISA KIT|Human is for detecting the concentration of Human BUD13 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of BUD13 ELISA KIT|Human
The microtiter plate provided in BUD13 ELISA KIT|Human has been pre-coated with an Human BUD13 antibody specific to Human BUD13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human BUD13antibody preparation specific for Human BUD13 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human BUD13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human BUD13in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that BUD13 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!