ENAM ELISA KIT|Human
Catalog EF004896
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
ENAM ELISA KIT Sensitivity< 0.094ng/ml
Application ENAM ELISA KIT|Human is for detecting the concentration of Human ENAM in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of ENAM ELISA KIT|Human
The microtiter plate provided in ENAM ELISA KIT|Human has been pre-coated with an Human ENAM antibody specific to Human ENAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human ENAMantibody preparation specific for Human ENAM then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ENAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human ENAMin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that ENAM ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!