Human 11-Dehydrothromboxane B2,11-dehydro-TXB2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human 11-dehydro-TXB2 ELISA KIT ,Human 11DHTXB2 ELISA KIT ,Human 11-Dehydrothromboxane B2 ELISA KIT ,Human 11-Dehydro-thromboxane B2 ELISA KIT

Intended use

Human 11-dehydro-TXB2 ELISA kit allows for the in vitro quantitative determination of Human 11- Dehydrothromboxane B2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Human 11-dehydro-TXB2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-dehydro-TXB2, During the reaction, 11-dehydro-TXB2 in the sample or standard competes with a fixed amount of biotin-labeled 11-dehydro-TXB2 for sites on a pre-coated Monoclonal antibody specific to 11-dehydro-TXB2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 11-dehydro-TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human 11-dehydro-TXB2 ELISA kit Range 15.6-1000pg/ml

Human 11-dehydro-TXB2 ELISA kit manual download

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