• Model: EF006079
  • 100 Units in Stock
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Catalog  EF006079

Packing 48T/96T

Reactivity Human

Detect Range 0.781-50ng/ml

12-HETE ELISA KIT Sensitivity< 0.469ng/ml

Application 12-HETE ELISA KIT|Human  is for detecting the concentration of Human 12-HETE in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade


Principle of 12-HETE ELISA KIT|Human

The microtiter plate provided in 12-HETE ELISA KIT|Human has been pre-coated with an Human 12-HETE antibody specific to Human 12-HETE . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human 12-HETE antibody preparation specific for Human 12-HETE then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 12-HETE , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human 12-HETE in the samples is then determined by comparing the O.D. of the samples to the standard curve.


*Please noted that 12-HETE ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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