Human 5-hydroxytryptamine receptor 2A, Htr2a ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human HTR2A ELISA KIT ,Human 5-HT2A ELISA KIT ,Human HTR2 ELISA KIT ,Human 5-HT2 receptor ELISA KIT ,Human 5-hydroxytryptamine (serotonin) receptor 2A ELISA KIT ,Human G protein-coupled ELISA KIT ,Human serotonin 5-HT-2A receptor ELISA KIT ,Human 5-hydroxytryptamine receptor 2A ELISA KIT

Intended use

Human Htr2a ELISA kit allows for the in vitro quantitative determination of Human Htr2a concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human Htr2a ELISA kit has been pre-coated with an antibody specific to Htr2a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Htr2a and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Htr2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Htr2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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