Human Acetylcholinesterase antibody ELISA Kit
Product Name:Human Acetylcholinesterase antibody ELISA Kit
Packing:96T
Catalog No.:ELA-E1447h
Gene Name:AChE.Ab
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Acetylcholinesterase antibody
Alternative Name:AChE.Ab,Acetylcholinesterase antibody
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Acetylcholinesterase antibody ELISA Kit allows for the in vitro quantitative determination of Acetylcholinesterase antibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Acetylcholinesterase antibody ELISA Kit has been pre-coated with an Acetylcholinesterase antibody antibody specific to Acetylcholinesterase antibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Acetylcholinesterase antibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acetylcholinesterase antibody, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Acetylcholinesterase antibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!