Human ACHE ELISA Kit

Product Name:Human ACHE ELISA Kit
Packing:96T

Catalog No.:ELA-E1195h

Gene Name:ACHE

Detect Range:0.312-20 ng/mL

Sensitivity:0.1ng/mL

Target Protein Name:Human Acetylcholinesterase

Alternative Name:Acetylcholinesterase, AChE, ACHE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ACHE ELISA Kit allows for the in vitro quantitative determination of Human Acetylcholinesterase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ACHE ELISA Kit has been pre-coated with an ACHE antibody specific to Human Acetylcholinesterase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Acetylcholinesterase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Acetylcholinesterase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm