Human Acyl- coenzyme A thioesterase 11, ACOT11 ELISA KIT
Product Name:Human Acyl- coenzyme A thioesterase 11, ACOT11 ELISA KIT
Packing:96T
Catalog No.:ELI-35004h
Gene Name:ACOT11
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Acyl-coenzyme A thioesterase 11(ACOT11
Alternative Name:ACOT11,Acyl-coenzyme A thioesterase 11(ACOT11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Acyl- coenzyme A thioesterase 11, ACOT11 ELISA KIT allows for the in vitro quantitative determination of Acyl-coenzyme A thioesterase 11(ACOT11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Acyl- coenzyme A thioesterase 11, ACOT11 ELISA KIT has been pre-coated with an Acyl-coenzyme A thioesterase 11(ACOT11 antibody specific to Acyl-coenzyme A thioesterase 11(ACOT11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Acyl-coenzyme A thioesterase 11(ACOT11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Acyl-coenzyme A thioesterase 11(ACOT11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Acyl-coenzyme A thioesterase 11(ACOT11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!