Human Alpha-fetoprotein Lens culinaris agglutiin 3, AFP- L3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

AFPL3,Alpha-fetoprotein Lens culinaris agglutiin 3, AFP-L3; Alpha-Fetoprotein Lens culinaris agglutiin 3; Alpha-fetoprotein isoform; AFP-L3

Search name

Human AFPL3 ELISA KIT ,Human Alpha-fetoprotein Lens culinaris agglutiin 3 ELISA KIT ,Human AFP-L3 ELISA KIT ,Human Alpha-Fetoprotein Lens culinaris agglutiin 3 ELISA KIT ,Human Alpha-fetoprotein isoform ELISA KIT ,Human AFP-L3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Alpha-fetoprotein Lens culinaris agglutiin 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-fetoprotein Lens culinaris agglutiin 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-fetoprotein Lens culinaris agglutiin 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-fetoprotein Lens culinaris agglutiin 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-fetoprotein Lens culinaris agglutiin 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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