Human Aminoacylase 1 ELISA Kit

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  • Model: ELA-E2259h
  • 20 Units in Stock
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Human AMINOACYLASE 1  ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  AMINOACYLASE 1

Protein Name  AminoAcylase-1

Alternative Name

Human ACY-1 ELISA KIT ,Human aminoacylase 1 ELISA KIT ,Human aminoacylase-1 ELISA KIT ,Human ACY-1 ELISA KIT ,Human AMINOACYLASE 1 D ELISA KIT ,Human HEL-S-5 ELISA KIT ,Human N-acyl-L-amino-acid amidohydrolase ELISA KIT ,Human acylase ELISA KIT ,Human epididymis secretory protein Li 5 ELISA KIT

Intended use

Human AMINOACYLASE 1  ELISA KIT allows for the in vitro quantitative determination of AMINOACYLASE 1  concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Human AMINOACYLASE 1  ELISA KIT has been pre-coated with an AMINOACYLASE 1  antibody specific to AMINOACYLASE 1 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AMINOACYLASE 1  and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMINOACYLASE 1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMINOACYLASE 1  in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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