Human Angiopoietin- related protein 2, ANGPTL2 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Human ANGPTL2 ELISA KIT ,Human ARP2 ELISA KIT ,Human HARP ELISA KIT ,Human angiopoietin-like 2 ELISA KIT ,Human angiopoietin-like protein 2 ELISA KIT ,Human angiopoietin like 2 ELISA KIT ,Human ANGL2 ELISA KIT

Intended use

Human ANGPTL2 ELISA KIT allows for the in vitro quantitative determination of human Angiopoietin-related protein 2, ANGPTL2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human ANGPTL2 ELISA KIT has been pre-coated with an antibody specific to ANGPTL2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANGPTL2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ANGPTL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANGPTL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Human ANGPTL2 ELISA KIT Manual download

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