Human AP1M1 ELISA Kit

Product Name:Human AP1M1 ELISA Kit
Packing:96T

Catalog No.:ELA-E12358h

Gene Name:AP1M1

Detect Range:0.312-20 ng/mL

Sensitivity:0.12ng/ml

Target Protein Name:Human AP-1 complex subunit mu-1

Alternative Name:AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, Clathrin assembly protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human AP1M1 ELISA Kit allows for the in vitro quantitative determination of Human AP-1 complex subunit mu-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human AP1M1 ELISA Kit has been pre-coated with an AP1M1 antibody specific to Human AP-1 complex subunit mu-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human AP-1 complex subunit mu-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human AP-1 complex subunit mu-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human AP-1 complex subunit mu-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!