Human ATP- binding cassette sub- family B member 10, mitochondrial, ABCB10 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ABCB10,EST20237; M-ABC2; MTABC2; ABC transporter 10 protein; ATP-binding cassette sub-family B member 10 mitochondrial; ATP-binding cassette transporter 10; mitochondrial ATP-binding cassette 2; ATP-binding cassette sub-family B (MDR/TAP) member 10,

Search name

Human ABCB10 ELISA KIT ,Human EST20237 ELISA KIT ,Human M-ABC2 ELISA KIT ,Human MTABC2 ELISA KIT ,Human ABC transporter 10 protein ELISA KIT ,Human ATP-binding cassette sub-family B member 10 mitochondrial ELISA KIT ,Human ATP-binding cassette transporter 10 ELISA KIT ,Human mitochondrial ATP-binding cassette 2 ELISA KIT ,Human ATP-binding cassette sub-family B (MDR/TAP) member 10 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human ATP- binding cassette sub- family B member 10, mitochondrial, ABCB10 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ABCB10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ABCB10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ABCB10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ABCB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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