Human Bcl- 2- binding component 3, BBC3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

BBC3,PUMA,JFY-1; JFY1; bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; BCL2 binding component 3

Search name

Human BBC3 ELISA KIT ,Human PUMA ELISA KIT ,Human JFY-1 ELISA KIT ,Human JFY1 ELISA KIT ,Human bcl-2-binding component 3 ELISA KIT ,Human p53 up-regulated modulator of apoptosis ELISA KIT ,Human BCL2 binding component 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Bcl- 2- binding component 3, BBC3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to BBC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BBC3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BBC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BBC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.