• Model: EF008136
  • 100 Units in Stock
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Catalog  EF008136

Packing 48T/96T                                                        Manual

Reactivity Human

Detect Range 0.313-20ng/ml

BPGM ELISA KIT Sensitivity< 0.188ng/ml

Application Human BPGM ELISA KIT  is for detecting the concentration of Human Bisphosphoglycerate mutase in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade


Principle of Human BPGM ELISA KIT

The microtiter plate provided in Human BPGM ELISA KIT has been pre-coated with an Human BPGM antibody specific to Human BPGM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human BPGMantibody preparation specific for Human BPGM then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human BPGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human BPGMin the samples is then determined by comparing the O.D. of the samples to the standard curve.


*Please noted that Human BPGM ELISA KIT is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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