Human BRCA1 ELISA Kit

Product Name:Human BRCA1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0939h

Gene Name:BRCA1

Detect Range:0.312-20 ng/mL

Sensitivity:0.078 ng/mL

Target Protein Name:Human Breast cancer type 1 susceptibility protein

Alternative Name:Breast cancer type 1 susceptibility protein, RING finger protein 53, BRCA1, RNF53
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human BRCA1 ELISA Kit allows for the in vitro quantitative determination of Human Breast cancer type 1 susceptibility protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human BRCA1 ELISA Kit has been pre-coated with an BRCA1 antibody specific to Human Breast cancer type 1 susceptibility protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Breast cancer type 1 susceptibility protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Breast cancer type 1 susceptibility protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm