Human C- X- C motif chemokine 16, CXCL16 ELISA KIT
Product Name:Human C- X- C motif chemokine 16, CXCL16 ELISA KIT
Packing:96T
Catalog No.:ELI-02636h
Gene Name:CXCL16
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:C-X-C motif chemokine 16(CXCL16
Alternative Name:CXCL16,C-X-C motif chemokine 16(CXCL16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C- X- C motif chemokine 16, CXCL16 ELISA KIT allows for the in vitro quantitative determination of C-X-C motif chemokine 16(CXCL16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C- X- C motif chemokine 16, CXCL16 ELISA KIT has been pre-coated with an C-X-C motif chemokine 16(CXCL16 antibody specific to C-X-C motif chemokine 16(CXCL16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C-X-C motif chemokine 16(CXCL16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-X-C motif chemokine 16(CXCL16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of C-X-C motif chemokine 16(CXCL16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!