Human Cadherin-2, CDH2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cadherin-2,CDw325,Neural cadherin,N-cadherin, N-cadherin,cadherin-2; CD325; CDHN; CDw325; NCAD; N-cadherin 1; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; neural cadherin; neural-cadherin; cadherin 2, type 1, N-cadherin neuronal

Search name

Human Cadherin-2 ELISA KIT ,Human CDw325 ELISA KIT ,Human Neural cadherin ELISA KIT ,Human N-cadherin ELISA KIT ,Human  N-cadherin ELISA KIT ,Human cadherin-2 ELISA KIT ,Human CD325 ELISA KIT ,Human CDHN ELISA KIT ,Human CDw325 ELISA KIT ,Human NCAD ELISA KIT ,Human N-cadherin 1 ELISA KIT ,Human cadherin 2 ELISA KIT ,Human  N-cadherin (neuronal) ELISA KIT ,Human calcium-dependent adhesion protein ELISA KIT ,Human  neuronal ELISA KIT ,Human neural cadherin ELISA KIT ,Human neural-cadherin ELISA KIT ,Human cadherin 2 ELISA KIT ,Human  type 1 ELISA KIT ,Human  N-cadherin neuronal ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Cadherin-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cadherin-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cadherin-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cadherin-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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