Human Carboxypeptidase E,CPE ELISA KIT

Starting at: $696.00

  • Model: ELI-50203h
  • 20 Units in Stock
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Human Carboxypeptidase E, CPE ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

CPE,carboxypeptidase E; CPH; carboxypeptidase H; cobalt-stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule-associated carboxypeptidase; prohormone-processing carboxypeptidase

 

Search name

Human CPE ELISA KIT ,Human carboxypeptidase E ELISA KIT ,Human CPH ELISA KIT ,Human carboxypeptidase H ELISA KIT ,Human cobalt-stimulated chromaffin granule carboxypeptidase ELISA KIT ,Human enkephalin convertase ELISA KIT ,Human insulin granule-associated carboxypeptidase ELISA KIT ,Human prohormone-processing carboxypeptidase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Carboxypeptidase E, CPE concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CPE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CPE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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