CCL8 ELISA KIT|Human
Catalog EF000056
Packing 48T/96T
Reactivity Human
Detect Range 6.25-400pg/ml
CCL8 ELISA KIT Sensitivity< 3.75pg/ml
Application CCL8 ELISA KIT|Human is for detecting the concentration of Human CCL8 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of CCL8 ELISA KIT|Human
The microtiter plate provided in CCL8 ELISA KIT|Human has been pre-coated with an Human CCL8 antibody specific to Human CCL8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human CCL8antibody preparation specific for Human CCL8 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human CCL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human CCL8in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that CCL8 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!