Human Centrosomal protein of 44 kDa, CEP44 ELISA KIT
Product Name:Human Centrosomal protein of 44 kDa, CEP44 ELISA KIT
Packing:96T
Catalog No.:ELI-34114h
Gene Name:CEP44
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Centrosomal protein of 44 kDa(CEP44
Alternative Name:CEP44,Centrosomal protein of 44 kDa(CEP44
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Centrosomal protein of 44 kDa, CEP44 ELISA KIT allows for the in vitro quantitative determination of Centrosomal protein of 44 kDa(CEP44 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Centrosomal protein of 44 kDa, CEP44 ELISA KIT has been pre-coated with an Centrosomal protein of 44 kDa(CEP44 antibody specific to Centrosomal protein of 44 kDa(CEP44 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centrosomal protein of 44 kDa(CEP44 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centrosomal protein of 44 kDa(CEP44, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centrosomal protein of 44 kDa(CEP44 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!