Human Cerberus,CER1 ELISA KIT

Starting at: $696.00

  • Model: ELI-50035h
  • 20 Units in Stock
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Human CER1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  CER1

Protein Name  Cerberus

Alternative Name

Human CER1 ELISA KIT ,Human DAND4 ELISA KIT ,Human cerberus ELISA KIT ,Human DAN domain family member 4 ELISA KIT ,Human cerberus 1 ELISA KIT ,Human  cysteine knot superfamily ELISA KIT ,Human  homolog ELISA KIT ,Human cerberus-related 1 ELISA KIT ,Human cerberus-related protein ELISA KIT ,Human cerberus 1 ELISA KIT ,Human DAN family BMP antagonist ELISA KIT

Intended use

Human CER1 ELISA KIT allows for the in vitro quantitative determination of CER1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Human CER1 ELISA KIT has been pre-coated with an CER1 antibody specific to CER1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CER1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CER1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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