Human Coagulation factor X, F10 ELISA KIT

Product Name:Human Coagulation factor X, F10 ELISA KIT
Packing:96T

Catalog No.:ELI-02818h

Gene Name:F10

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Coagulation factor X(F10

Alternative Name:F10,Coagulation factor X(F10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Coagulation factor X, F10 ELISA KIT allows for the in vitro quantitative determination of Coagulation factor X(F10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Coagulation factor X, F10 ELISA KIT has been pre-coated with an Coagulation factor X(F10 antibody specific to Coagulation factor X(F10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Coagulation factor X(F10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Coagulation factor X(F10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Coagulation factor X(F10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!