Human Collagen Type II Cleavage ELISA KIT

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  • Model: ELA-E2183h
  • 20 Units in Stock
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Human C2C ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  C2C

Protein Name  Collagen Type II Cleavage

Alternative Name

Human C2C ELISA KIT ,Human COL2 ELISA KIT ,Human Collagen Type II Cleavage ELISA KIT ,Human type II collagen ELISA KIT ,Human ANFH ELISA KIT ,Human AOM ELISA KIT ,Human COL11A3 ELISA KIT ,Human SEDC ELISA KIT ,Human STL1 ELISA KIT ,Human collagen alpha-1(II) chain ELISA KIT ,Human alpha-1 type II collagen ELISA KIT ,Human arthroophthalmopathy progressive (Stickler syndrome) ELISA KIT ,Human cartilage collagen ELISA KIT ,Human chondrocalcin ELISA KIT ,Human collagen II alpha-1 polypeptide ELISA KIT ,Human collagen type II alpha 1 ELISA KIT ,Human collagen type II alpha 1 chain ELISA KIT

 

Intended use

Human C2C ELISA KIT allows for the in vitro quantitative determination of C2C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Human C2C ELISA KIT has been pre-coated with an C2C antibody specific to C2C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C2C and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C2C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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