Human COQ10B ELISA Kit

Product Name:Human COQ10B ELISA Kit
Packing:96T

Catalog No.:ELA-E2074h

Gene Name:COQ10B

Detect Range:0.312-20 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial

Alternative Name:Coenzyme Q-binding protein COQ10 homolog B, mitochondrial, COQ10B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human COQ10B ELISA Kit allows for the in vitro quantitative determination of Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human COQ10B ELISA Kit has been pre-coated with an COQ10B antibody specific to Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!