Human Cortisol ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Cortisol,hydrocortisone,

Search name
Human Cortisol ELISA Kit,Human hydrocortisone ELISA Kit,Cortisol ELISA Kit,hydrocortisone ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general cortisol,Cortisol concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Cortisol, During the reaction, Cortisol in the sample or standard competes with a fixed amount of
biotin-labeled Cortisol for sites on a pre-coated Monoclonal antibody specific to Cortisol. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Cortisol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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