Human CYP7B1 ELISA Kit

Starting at: $696.00

  • Model: ELI-32312h
  • 20 Units in Stock
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Human 25-hydroxycholesterol 7-alpha-hydroxylase, Cyp7b1 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

 

Alternative name

Human Cyp7b1 ELISA KIT ,Human CBAS3 ELISA KIT ,Human CP7B ELISA KIT ,Human SPG5A ELISA KIT ,Human cytochrome P450 7B1 ELISA KIT ,Human cytochrome P450 family 7 subfamily B polypeptide 1 ELISA KIT ,Human cytochrome P450 subfamily VIIB (oxysterol 7 alpha-hydroxylase) polypeptide 1 ELISA KIT ,Human oxysterol 7-alpha-hydroxylase ELISA KIT ,Human cytochrome P450 family 7 subfamily B member 1 ELISA KIT ,Human 25-hydroxycholesterol 7-alpha-hydroxylase ELISA KIT ,Human Oxysterol 7-alpha-hydroxylase ELISA KIT ,Human Oxysterol 7-alpha-hydroxylase ELISA KIT ,Human Cytochrome P450 7B1 ELISA KIT ,Human Hippocampal transcript 1 protein ELISA KIT ,Human HCT-1 ELISA KIT

 

Intended use

Human Cyp7b1 ELISA kit allows for the in vitro quantitative determination of Human Cyp7b1 concenthumanions in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Human Cyp7b1 ELISA kit has been pre-coated with an antibody specific to Cyp7b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody prepahumanion specific for Cyp7b1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substhumane solution is added to each well. Only those wells that contain Cyp7b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substhumane reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concenthumanion of Cyp7b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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