Human cytokeratin 20, CK20 ELISA Kit

Starting at: $696.00

  • Model: ELA-E1691h
  • 20 Units in Stock
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Human cytokeratin 20, CK20 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Keratin, type I cytoskeletal 20,Protein IT,Cytokeratin-20,Keratin-20,KRT20,CK20; CD20; CK-20; K20; KRT21; cytokeratin 20; cytokeratin-20; keratin, type I cytoskeletal 20; keratin-20; protein IT; keratin 20

 

Search name

Human Keratin ELISA KIT ,Human  type I cytoskeletal 20 ELISA KIT ,Human Protein IT ELISA KIT ,Human Cytokeratin-20 ELISA KIT ,Human Keratin-20 ELISA KIT ,Human KRT20 ELISA KIT ,Human CK20 ELISA KIT ,Human CD20 ELISA KIT ,Human CK-20 ELISA KIT ,Human K20 ELISA KIT ,Human KRT21 ELISA KIT ,Human cytokeratin 20 ELISA KIT ,Human cytokeratin-20 ELISA KIT ,Human keratin type I cytoskeletal 20 ELISA KIT ,Human keratin-20 ELISA KIT ,Human protein IT keratin 20 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human keratin, type i cytoskeletal 20,Keratin, type I cytoskeletal 20 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin, type I cytoskeletal 20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Keratin, type I cytoskeletal 20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin, type I cytoskeletal 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Keratin, type I cytoskeletal 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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