Human Cytotoxic and regulatory T- cell molecule, CRTAM ELISA KIT

Product Name:Human Cytotoxic and regulatory T- cell molecule, CRTAM ELISA KIT
Packing:96T

Catalog No.:ELI-25754h

Gene Name:CRTAM

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Cytotoxic and regulatory T-cell molecule(CRTAM

Alternative Name:CRTAM,Cytotoxic and regulatory T-cell molecule(CRTAM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Cytotoxic and regulatory T- cell molecule, CRTAM ELISA KIT allows for the in vitro quantitative determination of Cytotoxic and regulatory T-cell molecule(CRTAM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Cytotoxic and regulatory T- cell molecule, CRTAM ELISA KIT has been pre-coated with an Cytotoxic and regulatory T-cell molecule(CRTAM antibody specific to Cytotoxic and regulatory T-cell molecule(CRTAM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytotoxic and regulatory T-cell molecule(CRTAM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytotoxic and regulatory T-cell molecule(CRTAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytotoxic and regulatory T-cell molecule(CRTAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!