Human DDO ELISA Kit

Product Name:Human DDO ELISA Kit
Packing:96T

Catalog No.:ELA-E1156h

Gene Name:DDO

Detect Range:0.312-20 ng/mL

Sensitivity:0.11ng/mL

Target Protein Name:Human D-aspartate oxidase

Alternative Name:D-aspartate oxidase, DASOX, DDO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human DDO ELISA Kit allows for the in vitro quantitative determination of Human D-aspartate oxidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human DDO ELISA Kit has been pre-coated with an DDO antibody specific to Human D-aspartate oxidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human D-aspartate oxidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human D-aspartate oxidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm