Human DNA topoisomerase 3- beta- 1, TOP3B ELISA KIT

Product Name:Human DNA topoisomerase 3- beta- 1, TOP3B ELISA KIT
Packing:96T

Catalog No.:ELI-40150h

Gene Name:TOP3B

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:DNA topoisomerase 3-beta-1(TOP3B

Alternative Name:TOP3B,DNA topoisomerase 3-beta-1(TOP3B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human DNA topoisomerase 3- beta- 1, TOP3B ELISA KIT allows for the in vitro quantitative determination of DNA topoisomerase 3-beta-1(TOP3B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human DNA topoisomerase 3- beta- 1, TOP3B ELISA KIT has been pre-coated with an DNA topoisomerase 3-beta-1(TOP3B antibody specific to DNA topoisomerase 3-beta-1(TOP3B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA topoisomerase 3-beta-1(TOP3B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA topoisomerase 3-beta-1(TOP3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA topoisomerase 3-beta-1(TOP3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!