Human Dual specificity protein phosphatase 2, DUSP2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DUSP2,PAC-1; PAC1; dual specificity protein phosphatase 2; dual specificity protein phosphatase PAC-1; dual-specificity phosphatase 2; serine/threonine specific protein phosphatase; dual specificity phosphatase 2

Search name

Human DUSP2 ELISA KIT ,Human PAC-1 ELISA KIT ,Human PAC1 ELISA KIT ,Human dual specificity protein phosphatase 2 ELISA KIT ,Human dual specificity protein phosphatase PAC-1 ELISA KIT ,Human dual-specificity phosphatase 2 ELISA KIT ,Human serine/threonine specific protein phosphatase ELISA KIT ,Human dual specificity phosphatase 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Dual specificity protein phosphatase 2, DUSP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Dual specificity protein phosphatase 2, DUSP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Dual specificity protein phosphatase 2, DUSP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Dual specificity protein phosphatase 2, DUSP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Dual specificity protein phosphatase 2, DUSP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.