Human E3 ubiquitin- protein ligase ZNRF1, ZNRF1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ZNRF1,E3 ubiquitin-protein ligase ZNRF1; NIN283; nerve injury gene 283; nerve injury-induced gene 283 protein; zinc and ring finger protein 1; zinc/RING finger protein 1; zinc and ring finger 1, E3 ubiquitin protein ligase
Search name
Human ZNRF1 ELISA KIT ,Human E3 ubiquitin-protein ligase ZNRF1 ELISA KIT ,Human NIN283 ELISA KIT ,Human nerve injury gene 283 ELISA KIT ,Human nerve injury-induced gene 283 protein ELISA KIT ,Human zinc and ring finger protein 1 ELISA KIT ,Human zinc/RING finger protein 1 ELISA KIT ,Human zinc and ring finger 1 ELISA KIT ,Human E3 ubiquitin protein ligase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human E3 ubiquitin- protein ligase ZNRF1, ZNRF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin- protein ligase ZNRF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for E3 ubiquitin- protein ligase ZNRF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin- protein ligase ZNRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 ubiquitin- protein ligase ZNRF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month